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ptsH [2019-02-08 18:07:07]

HPr, General component of the sugar [SW|phosphotransferase system] (PTS)

locus

BSU13900

4.58

9.05 kDa

protein length

88 aa Sequence Blast

gene length

267 bp Sequence Blast

function

PTS-dependent sugar transport and carbon catabolite repression

product

histidine-containing phosphocarrier protein HPr of the PTS

essential

2.7.11.-

synonyms

HPr

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

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categories

[category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.1|General PTS proteins]

[category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]

[category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

[category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

Gene

Coordinates

1,459,384 1,459,650

The protein

Catalyzed reaction/ biological activity

Protein HPr N(pi)-phospho-L-histidine + protein EIIA = protein HPr + protein EIIA N(tau)-phospho-L-histidine (according to Swiss-Prot)

Protein family

HPr domain (according to Swiss-Prot) HPr family

Paralogous protein(s)

[protein|A269774F2FDC94F93BA5F1360FFFE754B50383AD|Crh]

[SW|Domains]

HPr Domain (288)

Modification

transient phosphorylation by [protein|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|Enzyme I] of the PTS on His-15

regulatory phosphorylation on Ser-46 by [protein|771F205F818A755A661B8E1C95365A4F6AEB05C7|HprK] [Pubmed|2507315]

an extensive study on ''in vivo'' HPr phosphorylation can be found in Singh et al. (2008) [PubMed|18757537]

weak phosphorylation on Ser-12 [Pubmed|17218307]

''in vitro'' phosphorylated by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|PrkC] on Ser-12 [Pubmed|20389117]

Structure

[PDB|2HID] (NMR) [Pubmed|9336834]

[PDB|1KKM] (complex of ''L. casei'' [protein|771F205F818A755A661B8E1C95365A4F6AEB05C7|HprK] with ''B. subtilis'' HPr-Ser-P)

[PDB|1KKL] (complex of ''Lactobacillus casei'' [protein|771F205F818A755A661B8E1C95365A4F6AEB05C7|HprK] with ''B. subtilis'' HPr)

[SW|Localization]

cytoplasm [Pubmed|16395550]

additional information

belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

genes

[gene|29B793660E4D30C0656248F3EF403FEF76FB9025|ptsH]-[gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]

description

[Pubmed|11902727]

sigma factors

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genes

description

[Pubmed|11902727]

sigma factors

regulatory mechanism

[regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

regulation

expression activated by glucose (2 fold) ([protein|search|GlcT]) [Pubmed|12850135]

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Biological materials

Mutant

available in [SW|Jrg Stlke]'s lab:

MZ303 (cat)

GP507 ptsH1 (S46A)

GP506 (ptsH-H15A)

BKE13900 ([gene|29B793660E4D30C0656248F3EF403FEF76FB9025|ptsH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGAATTGACCTCCTCT, downstream forward: _UP4_TAAGGGTGTTAGTACGCCGT

BKK13900 ([gene|29B793660E4D30C0656248F3EF403FEF76FB9025|ptsH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGAATTGACCTCCTCT, downstream forward: _UP4_TAAGGGTGTTAGTACGCCGT

Expression vectors

pGP438 (with N-terminal Strep-tag, in [SW|pGP172]), available in [SW|Jrg Stlke]'s lab

pAG2 (His-tag) [Pubmed|9237995], available in [SW|Anne Galinier] lab

pGP371(expression / purification of HPr-S46A, with His-tag from ''E. coli'', in [SW|pWH844]), available in [SW|Jrg Stlke]'s lab

pGP1415 (HPr, expression in ''B. subtilis'', from [SW|pBQ200]), available in [SW|Jrg Stlke]'s lab

pGP961 (HPr, expression in ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], available in [SW|Jrg Stlke]'s lab

pGP1416 (HPr-H15A, expression in ''B. subtilis'', from [SW|pBQ200]), available in [SW|Jrg Stlke]'s lab

pGP2431 (N-terminal Strep-tag, expression and purification from ''B. subtilis'', in [SW|pGP380]), for [SW|SPINE], available in [SW|Jrg Stlke]'s lab

two-hybrid system

''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jrg Stlke]'s lab

Antibody

available in [SW|Jrg Stlke]'s lab

labs

[SW|Josef Deutscher], Paris-Grignon, France

[SW|Jrg Stlke], University of Gttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

[SW|Richard Brennan], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]

[SW|Boris Grke], University of Gttingen, Germany [http://wwwuser.gwdg.de/~genmibio/goerke.html Homepage]

[SW|Anne Galinier], University of Marseille, France

References

16519689,12850135,17218307,16519689,17142398,12359875,1577686,9162046,11929549,9336834,7803390,7623661,2846556,8169206,9973552,15126459,10048041,12169607,9622354,10217795,17693724,18757537,9202047,7592487,15369672,14527945,2507315,8580838,19651770,8418852,26282429,1303754,1549615,20081037,20389117,20444094,22001508,22722928,23551403,15378759,26381121,9336834